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KMID : 0370220230670010045
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Yakhak Hoeji
2023 Volume.67 No. 1 p.45 ~ p.50
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?Cynara Scolymus L inhibits the LPS-induced inflammatory reaction via suppression of NF-κB activity in RAW 264.7 cells
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Boo Kyung-Jun
Lee Ki-Man
Park Il-Ho
Kang Tae-Jin
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Abstract
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Cynara scolymus L. (artichoke) has been traditionally used in the treatment of digestive-related disease, severehyperlipidemia and liver disease. Recently, anti-inflammatory effect of artichoke has been reported by several studies, butits mechanisms are still unclear. In this study, the anti-inflammatory mechanism of artichoke was studied using an in vitroacute inflammation model. The effect of artichoke on the expression of pro-inflammatory cytokines, such as interleukin-1beta (IL-1β), IL-6, and tumor necrosis factor-alpha (TNF-α) in murine macrophage cell line, RAW264.7, was examinedby using ELISA and RT-PCR. As a result, artichoke inhibited the expression of IL-1β, IL-6 and TNF-α in macrophagesactivated by lipopolysaccharide (LPS) at a dose dependent manner. The expression of inflammatory mediators such as NOand PGE2 was next investigated in RAW264.7 cells stimulated with LPS. Artichoke also inhibited the production of NOand PGE2 in the cells at a dose dependent manner. Artichoke suppressed the mRNA expression of iNOS and COX-2 inmacrophages by LPS. The effect of artichoke on the activation of NF-κB was examined and LPS-induced NF-κBactivation was decreased by treatment of artichoke, suggesting that artichoke has anti-inflammatory effect by inhibiting NF-κB activation.
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KEYWORD
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Cynara scolymus L., Artichoke, Inflammation, NF-κB
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